N6-adenosine-methyltransferase MTA70-like participates antiviral responses in <i>Nicotiana benthamiana</i>.

ABSTRACT:

Introduction

Previous studies have shown that m6A methylation has a certain regulatory role in the replication of human viruses and plant viruses. Particularly, the m6A methylation in the host endogenous gene significantly decreased after the infection of potato virus Y (PVY).

Methods

Here, we cloned and characterized N6-adenosine-methyltransferase MTA70-like (ToMTA70) from tomato. The ToMTA70 was constructed into the pMDC32 binary vector. To create the ToMTA70 mutant, sequence of the MTA70 superfamily domain (493-653) with PDS and GUS sequences, respectively. The effect of the ToMTA70 on viral accumulation was studied using an Agrobacterium-mediated transient expression assay.

Results

A conserved MT-A70 superfamily domain in ToMTA70 was identified by encoding sequenc

SUBMITTER: Wang H 

PROVIDER: S-EPMC12826071 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature