Integrative genomic analysis reveals DHX58 as a key player in gastric cancer.

ABSTRACT: Gastric cancer (GC) is a major global health burden with limited treatment options. Identifying the molecular mechanisms underlying GC progression is critical for developing novel therapeutic strategies. We integrated whole-genome bisulfite sequencing and bulk RNA sequencing to identify hub genes involved in GC. Functional annotations were performed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, supplemented by data from The Cancer Genome Atlas. Key findings were validated using western blotting, immunohistochemistry, quantitative real-time PCR, and methylation-specific PCR. DExH-box helicase 58 (DHX58) was identified as a hypomethylated, highly expressed hub gene in GC. Mechanistically, DHX58 expression is regulated by the transcription factor CCAAT e

SUBMITTER: Li Y 

PROVIDER: S-EPMC12826466 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature