Expanding the Horizons of UV-vis Spectroscopy Education: Beyond the Beer-Lambert Law.

ABSTRACT: UV-visible (UV-vis) spectrophotometry is a fundamental analytical technique widely used in chemistry, biology, materials science, and medical research. Despite its significance, undergraduate education often presents UV-vis spectroscopy narrowly as an absorbance-based method governed by the Beer-Lambert Law. This traditional framework, while effective for idealized solutions, fails to address the complexities of real-world samples that scatter or emit light, leading to widespread challenges in data interpretation. To bridge this gap, this work introduces an expanded pedagogical approach that incorporates the theoretical foundations of UV-vis spectroscopy for solutions exhibiting diverse optical behaviors. These include pure absorbers, pure scatterers, simultaneous absorbers and scatterers,

SUBMITTER: Wathudura P 

PROVIDER: S-EPMC12826546 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature