A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain.

ABSTRACT: Uncovering protein interaction networks in vivo is essential for understanding physiological and pathological processes. Here, we report the generation of a novel knock-in mouse model expressing miniTurbo, a highly active biotin ligase, fused to the endogenous Usp46 gene. This model enables proximity-dependent biotin labeling (BioID) of USP46-associated proteins in the brain. In adult mice, biotinylation was induced by feeding a 0.1% biotin diet. We further evaluated whether the combination of miniTurbo and dietary biotin supplementation is effective for BioID in the developing brain. Biotinylation was successfully induced in embryonic and neonatal brains via maternal biotin intake, demonstrating the transfer of biotin to the offspring through the placenta during pregnancy and through milk

SUBMITTER: Murata K 

PROVIDER: S-EPMC12826572 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature