Diagnostic value of multi-gene methylation in colorectal cancer screening.

ABSTRACT:

Objective

To evaluate the diagnostic value and clinical application of multi-gene methylation assay in colorectal cancer (CRC) screening.

Methods

This was a single-center, retrospective, real-world study conducted at the First Affiliated Hospital of Shantou University Medical College, China. A total of 450 participants were enrolled from January 2023 to December 2024, including 150 healthy individuals, 120 colorectal polyp patients, and 180 CRC patients. The methylation status of six plasma-derived loci (SEPT9-R1, SEPT9-R2, BCAT1, IKZF1, BCAN, and VAV3) was assessed using real-time multiplex quantitative PCR (qPCR). The diagnostic performance and predictive factors of the multi-gene methylation assay for CRC detection were assessed.

Results

The multi-gene methylation

SUBMITTER: Yang Q 

PROVIDER: S-EPMC12827121 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature