A twin xanthan lyase-dependent xanthan degradation system in Paenibacillus taichungensis I5.

ABSTRACT: Xanthan gum, a natural heteropolysaccharide produced by Xanthomonas species, has many biotechnological applications across industries due to its unique rheological properties. Expanding its utility requires specific enzymes capable of targeted xanthan modification or degradation. In this study, a novel bacterial strain, isolated from a spoiled xanthan sample and identified as Paenibacillus taichungensis I5, was shown to degrade xanthan using a plate screening assay with Congo red. Activity tests of crude enzyme in culture supernatant demonstrated the secretion of xanthan-degrading enzymes. Genome and proteome analyses suggest a chromosomal xanthan utilization locus encoding a suite of enzymes, including a xanthanase (Pt_XanGH9), two xanthan lyases (Pt_XanPL8a and Pt_XanPL8b), two unsaturat

SUBMITTER: Han R 

PROVIDER: S-EPMC12827396 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature