A Zdhhc22-mCherry BAC transgenic mouse line reveals neuronal expression dynamics in the developing and adult CNS.

ABSTRACT: The DHHC palmitoyltransferase Zdhhc22 is known to play a role in neuronal differentiation, synaptic regulation, and brain development. While transcriptomic data hint at region-specific expression, its exact spatiotemporal and cell-type distribution in the mammalian brain is unclear. For this purpose, we generated a bacterial artificial chromosome (BAC) transgenic mouse line that expresses the mCherry fluorescent reporter driven by the Zdhhc22 promoter. We then analyzed Zdhhc22 expression from embryonic day 13.5 (E13.5) through adulthood. mCherry fluorescence was detected in many brain regions, including the cortex, thalamus, midbrain, piriform cortex, and brainstem. Interestingly, a dynamic developmental gene expression pattern was observed: Zdhhc22 expression was initially restricted to t

SUBMITTER: Yang H 

PROVIDER: S-EPMC12827437 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature