Making deep immunophenotyping accessible: the successful application of a guided 23-parameter mouse immunophenotyping panel package provided through a shared resource.

ABSTRACT: This 23-color mouse immunophenotyping panel was designed and developed by the Virginia Commonwealth University's (VCU) flow cytometry shared resource (FCSR) to easily bring new use to our high-parameter spectral cytometers. Our method is broadly applicable to multiple tissue types, is modifiable, and provides a reproducible, cost-effective option for utilizing high-parameter flow cytometry. To facilitate the mouse immunophenotyping panel, researchers can be provided with optimized reagents, a step-by-step staining protocol, instrument training, pre-run single-color controls, and acquisition and analysis templates to streamline the workflow. Data analysis is generally done with a traditional manual gating strategy, but t-stochastic neighbor embedding (tSNE) and uniform manifold approximatio

SUBMITTER: Isbell MG 

PROVIDER: S-EPMC12827670 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature