Short cold atmospheric plasma treatment preserves vascular quiescence in 3D tumor-stroma-endothelial models of pancreatic cancer in vitro and in ovo.

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) features a dense desmoplastic stroma, limiting drug delivery and promoting chemoresistance. While anti-angiogenic strategies have shown limited success, underexplored pro-angiogenic approaches may improve perfusion and treatment efficacy. Cold atmospheric plasma (CAP) generates reactive oxygen and nitrogen species and demonstrates anti-cancer effects at longer treatments and pro-healing, angiogenic effects at shorter treatments. We investigated the impact of short CAP treatment on angiogenesis in PDAC using a triple co-culture spheroid model and a turkey in ovo model, mimicking the tumor microenvironment. CAP was applied via the kINPen MED for 15-60 s in vitro and 10-30 s in ovo. In vitro, CAP inhibited endothelial tube formation treatment time-depen

SUBMITTER: Verloy R 

PROVIDER: S-EPMC12827977 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature