Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of Live <i>Salmonella enterica</i> and <i>Cronobacter sakazakii</i>.

ABSTRACT: Viability PCR (v-PCR) was optimized using propidium monoazide (PMA) and Triton X-100 for the selective detection of live foodborne pathogens in this study. The colony-forming unit (CFU) method, conventionally used for detecting live foodborne pathogens, is time-consuming. Quantitative PCR (qPCR) and droplet digital PCR (ddPCR) have emerged as rapid alternatives for pathogen detection, which provide accurate detection at low bacterial concentrations. However, these methods cannot distinguish between live and dead bacteria. We investigated the optimization of v-PCR conditions using PMA concentrations (10-200 μM) and Triton X-100 concentrations (0-1%) for Salmonella enterica subsp. enterica and Cronobacter sakazakii. PMA at 100 μM effectively inhibited the amplification o

SUBMITTER: Park M 

PROVIDER: S-EPMC12828127 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature