Enhancement of levopimaradiene production in <i>Escherichia coli</i> via engineering isopentenol utilization pathway.

ABSTRACT:

Objective

Plant-derived terpenoid ginkgolides exhibit significant pharmacological efficacy, however, their extraction remains costly. Given that levopimaradiene is a key biosynthetic precursor to ginkgolides, its high-yield production via heterologous gene expression therefore establishes a critical foundation for scaling up their manufacture. This study primarily aims to enhance the yield of levopimaradiene in Escherichia coli (LB medium) by remodeling the unnatural isopentenol utilization pathway (IUP).

Methods

Plasmid construction was driven by the mechanism of homologous recombination, which utilizes recombinase to facilitate the ligation process. The expression of kinase was carried out using type 7 (T7) promoter and isopropyl β-D-1-thiogalactopyran

SUBMITTER: Jia C 

PROVIDER: S-EPMC12828142 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature