Dissecting Heterogeneity Reveals Functional Subpopulation of Stem Cells From Human Exfoliated Deciduous Teeth.

ABSTRACT:

Objectives

Identify the functional subpopulation's surface marker of stem cells from human exfoliated deciduous teeth (SHEDs) using single-cell RNA sequencing (scRNA-seq) and isolate the subset, providing a theoretical basis for the refined application of stem cells.

Methods

scRNA-seq identified SHEDs as distinct subpopulations. GO and KEGG analyses were performed to define each subpopulation. Pseudotime trajectory analysis was employed to elucidate the potential differentiation processes of SHEDs. To investigate the multipotent differentiation potential of the BAMBI⁺ subpopulation, we conducted qPCR, Western blot analysis, Alizarin Red staining, Oil Red O staining, Alcian Blue staining, and immunohistochemical staining. The angiogenic capacity of the BAMBI^+<

SUBMITTER: Peng Y 

PROVIDER: S-EPMC12828145 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature