Genome-wide identification and characterization of methyltransferase involved in benzylisoquinoline alkaloids biosynthesis from <i>Menispermum dauricum</i>.

ABSTRACT:

Objective

Benzylisoquinoline alkaloids (BIAs) are valuable plant metabolites whose structural diversity largely depends on O-/N-methyltransferases (OMTs/NMTs). Although the CYP450-mediated backbone formation of BIAs was previously elucidated in Menispermum dauricum DC., the specific OMTs/NMTs responsible for their functional methylation remain unknown. This study aims to systematically identify and characterize these methyltransferases to define their roles in BIA biosynthesis.

Methods

Combining genomic and biochemical approaches, a genome-wide identification of methyltransferase (MT) genes was conducted using the published M. dauricum genome. The candidate MTs were functionally evaluated through in vitro enzymatic assays employing diverse

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12828156 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature