A Simple Programmable Cas12a/crRNA Induced Walking System for Sensitive Methicillin-Resistant <i>Staphylococcus aureus</i> Detection via Integrated <i>cis</i>- and <i>trans</i>-Cleavage Activity.

ABSTRACT: Methicillin-resistant Staphylococcus aureus (MRSA) represents a serious threat to public health due to its strong antibiotic resistance, wide dissemination, and high infection rates. Rapid identification of MRSA strains is essential for accurate diagnosis and timely treatment of related infections. In this study, we propose an analytical method for MRSA that employs a hairpin-structured locker-probe to directly regulate the trans-cleavage activity of Cas12a. This designed locker-probe connects a target-specific aptamer to an inhibitory aptamer of the CRISPR/Cas12a system. Upon binding to the specific target, the probe undergoes a conformational change that abolishes its inhibitory effect on Cas12a. As a result, the structure-switchable probe modulates Cas12a activity in a tar

SUBMITTER: Xiao B 

PROVIDER: S-EPMC12828325 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature