Rif-seq reveals Caulobacter crescentus mRNA decay is globally coordinated with transcription and translation.

ABSTRACT: While transcription and translation have been shown to be coordinated with mRNA decay across various single-gene studies, their global coordination remains poorly defined. Therefore, we utilize rifampicin sequencing experiments in Caulobacter crescentus to measure genome-wide mRNA lifetimes and analyze the impact of transcription and translation. Based on the RNA polymerase elongation speed, we identify that approximately 20% of the transcriptome is cotranscriptionally degraded. To investigate translation's impact on mRNA decay, we find that translation efficiency measured by ribosome profiling correlates with mRNA lifetime. We compare the 5' P cleavage sites to ribosome occupancy and find that cleavage sites occur preferentially in regions of low ribosome occupancy. Using the translation

SUBMITTER: Dilrangi KH 

PROVIDER: S-EPMC12828775 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature