Association of D516V, H526Y, and S531L rpoB gene polymorphisms and risk factors with rifampicin resistance in Mycobacterium tuberculosis isolates from pulmonary TB patients in Northwest Amhara, Ethiopia: cross-sectional study.

ABSTRACT:

Background

Drug-resistant tuberculosis (DR-TB) continues to pose a threat to public health worldwide. Rifampicin (RIF) resistance is mostly caused by mutations in the rpoB gene, which codes for the β -subunit of RNA polymerase and is also an important surrogate marker for multidrug-resistant tuberculosis (MDR).

Objective

This study aimed to detect the rpoB gene mutations associated with RIF resistance and identify the risk factors for MDR/ RIF resistance patterns in individuals infected with pulmonary TB.

Methods

A facility-based cross-sectional study was conducted at selected TB treatment center hospitals (Felegehiwot, Debre-tabor, University of Gondar, Debark, and Metema hospitals) from June to December 2023 in the Northwestern Amhara regional state of Ethiopia. A t

SUBMITTER: Getachew K 

PROVIDER: S-EPMC12828965 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature