Resilience of human tolerogenic dendritic cells to physiological oxygen supports clinical application: functional stability amidst glycolytic and differentiation shifts.

ABSTRACT: Tolerogenic dendritic cells (tolDCs) hold promise for treating autoimmune diseases, potentially restoring antigen-specific immune tolerance without systemic immunosuppression. However, their behavior in vivo remains incompletely understood, particularly in response to microenvironmental factors such as oxygen (O₂) tension. While tolDCs are typically generated and functionally validated under atmospheric O₂ (21%), physiological O₂ levels (physioxia) in human tissues are considerably lower (3-9%). The primary aim of this study was to assess whether tolDCs manufactured under atmospheric O₂ conditions retain their function under physioxia at 4% O₂, mimicking tissue environments encountered upon clinical administration. To contextualize the

SUBMITTER: Peter A 

PROVIDER: S-EPMC12829039 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature