Effect of free fatty acids on TGF-β1 mediated fibrogenesis in hepatic stellate cells.

ABSTRACT:

Abstract/objective

Metabolic associated steatotic liver disease (MASLD) is the most prevalent liver disorder and a major risk factor for hepatic fibrosis. Activated hepatic stellate cells (HSCs) are the primary source of collagen production in the liver, contributing to fibrosis. However, the mechanisms by which HSCs reprogram their metabolism to support sustained collagen production, particularly in a lipid-rich environment such as MASLD, remain inadequately understood. In this study, we investigated the effect of extracellular fatty acids on HSC substrate metabolism, HSC activation, and collagen synthesis.

Methods

Immortalized human HSCs (LX-2 cells) were cultured with or without transforming growth factor-beta 1 (TGF-β1) and varying concentrations of palmitate or oleate.

SUBMITTER: De Nardo W 

PROVIDER: S-EPMC12829130 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature