Screening and characterization of monoclonal antibodies with neutralizing activity against porcine transmissible gastroenteritis virus.

ABSTRACT:

Background

Porcine Transmissible Gastroenteritis Virus (TGEV) is widespread and frequently involved in co-infections, leading to high mortality rates in piglets and significantly constraining the swine industry. Based on the genetic sequence of TGEV strain HB-1, we designed and constructed a eukaryotic expression plasmid, pCAGGS-TGEV-CTD, encoding the CTD gene of TGEV. The recombinant CTD protein was successfully expressed and purified using the HEK-293 F suspension cell expression system. Balb/c mice were immunized with the purified CTD protein.

Results

Upon achieving a serum antibody titer of 10⁵, antigen-specific memory B cells were isolated using single-cell flow cytometry sorting. A total of 83 matched antibody light- and heavy-chain gene pairs were obtained via single-

SUBMITTER: Jia H 

PROVIDER: S-EPMC12829172 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature