Interactions between Plasmodium falciparum-infected red blood cells and their extracellular vesicles with megakaryocytes: implications for platelet-like particle formation.

ABSTRACT:

Background

Thrombocytopenia commonly accompanies Plasmodium falciparum (P. falciparum) malaria, yet the role of impaired megakaryopoiesis and platelet production remains unclear. This study examined how P. falciparum-infected red blood cells (pRBCs) and their extracellular vesicles (EVs) modulate megakaryocytic differentiation and platelet-like particle (PLP) formation.

Methods

MEG-01 cell line, a human megakaryoblastic leukaemia model, was differentiated with phorbol 12-myristate 13-acetate (PMA) in the presence or absence of fetal bovine serum (FBS) and co-cultured with pRBCs, normal RBCs (nRBCs), or their EVs (nRBC-EVs/pRBC-EVs). Changes in cell phenotype, adhesion, and gene expression were analyzed by flow cytometry, confocal microscopy, and qPCR. PLP functionality was a

SUBMITTER: Nunthanasup N 

PROVIDER: S-EPMC12829250 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature