Modeling kidney fibrosis and tubular regeneration in iPSC-derived kidney organoids.

ABSTRACT:

Background

Kidney fibrosis is one of the pathological hallmarks of chronic kidney disease, likely contributing to the loss of kidney function. The mechanisms leading to kidney fibrosis and its reversibility is only partially understood, which hampers the development of therapeutic targets. Therefore, it is crucial to establish a robust human in vitro model that can be used to study kidney fibrosis and potential regeneration.

Methods

Human induced pluripotent stem cells (iPSC) were differentiated into kidney organoids. Fibrotic injury was induced by mimicking hypoxia (1% O₂ 48 h), inflammation (interleukin-1 beta (IL-1β) 96 h) or a combination (hypoxia and IL-1β). Organoids were harvested at injury onset and up to 2 weeks post-injury. Fibrosis was assessed by mRNA

SUBMITTER: Li S 

PROVIDER: S-EPMC12829276 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature