Exploring the changes in anticoagulant active ingredients before and after bovine bile fermentation of the traditional Chinese medicine Yaomu <i>via</i> an aggregation-induced emission fluorescence sensor.

ABSTRACT: Fermentation is a significant and specialized processing technology that primarily serves to enhance efficacy and reduce toxicity. Yaomu (YM) was fermented in a cellar using bovine bile as the fermentation matrix. However, the alterations in its active ingredients before and after processing remain poorly understood. An aggregation-induced emission fluorescence sensor (NITPA-S) was developed that employed S-2238 (S) to encapsulate the NITPA-Py-603 (NITPA) probe with the help of glutaraldehyde, creating an "ON" state. Thrombin hydrolyzed the sensor, releasing the probe in the "OFF" state, while thrombin inhibitors reversed this effect. We combined an HPLC fully automatic partial fraction collector and UHPLC-Q-TOF/MS for the separation, screening and identification of YM active compounds, wh

SUBMITTER: Qin Q 

PROVIDER: S-EPMC12829439 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature