A Novel Link Between Electron Transport Chain Modulation and mcl-PHA Production in Pseudomonas aeruginosa 5300 Using Azide as a Modulator.

ABSTRACT: Medium chain length polyhydroxyalkanoates (mcl-PHA) are elastomeric biodegradable bioplastics produced by a few bacteria. The large-scale mcl-PHA production remains limited by the low yield of bacterial strains and biomass reduction due to prolonged nutrient limitation in the media. In the present study, Pseudomonas aeruginosa MCC 5300 produced mcl-PHA copolymer of about 39.4% cell dry weight (CDW) at a short duration of 24 h of growth in tryptic soy broth media containing oleic acid and the PHA content was enhanced up to 66.4% CDW in the presence of azide. The production of mcl-PHA at such a short duration was not reported previously in nutrient-enriched conditions. The application of azide enhanced mcl-PHA production in the bacteria. Oleic acid shifted the electron transport chain (ETC)

SUBMITTER: Paduvari R 

PROVIDER: S-EPMC12829472 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature