Evaluation of rat pancreatic islets damage after siRNA microporation.

ABSTRACT: Small interfering RNA (siRNA) can be used for the temporary inhibition of gene expression, and one possible delivery method is microporation. In this study, we evaluated whether microporation is a suitable technique for the transfection of pancreatic islets and whether the selected siRNA is recognized by islet cells as foreign, potentially triggering an inflammatory or cytotoxic response. Rat islets were transfected with siRNA by microporation 24 hours after isolation. Another 24 hours post-transfection, the islets were assessed for viability, function, and the expression of inflammatory markers. Microporation induced mild but, in some assays, statistically significant stress. Flow cytometry revealed 9.94% dead cells in the negative control, 16.33% in islets microporated without siRNA, and

SUBMITTER: Tomsovska V 

PROVIDER: S-EPMC12829790 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature