GC-MS based bioactive profiling of Phyllanthus niruri and its antibacterial potential through experimental and computational studies.

ABSTRACT:

Objective

The study aimed to evaluate the antibacterial potential of Phyllanthus niruri by identifying its bioactive compounds through GC-MS, assessing their in vitro antibacterial efficacy, and validating their interactions with bacterial target proteins through molecular docking and pharmacokinetic analyses.

Methods

Methanolic extracts were prepared and fractionated into petroleum ether (PSF), chloroform (CSF), carbon tetrachloride (CTF), ethyl acetate (ESF), methanol (MSF), and aqueous (AQF) fractions using the Kupchan method. Phytochemical screening, total phenolic content (TPC), and total flavonoid content (TFC) were determined spectrophotometrically. GC-MS analysis identified volatile constituents in the methanol extract. Antibacterial activity was evaluated against ni

SUBMITTER: Labu ZK 

PROVIDER: S-EPMC12829960 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature