Optimization of culture conditions for enhanced antibacterial metabolite production by Bacillus velezensis TL and evaluation of its efficacy against necrotic enteritis in broilers.

ABSTRACT: Bacillus velezensis has been widely utilized in agriculture and livestock; however, the efficacy of the metabolites of B. velezensis in the prevention and control of animal disease has not been reported. This study aimed to increase the production of antibacterial metabolites of B. velezensis TL by optimizing the culture conditions and evaluating their ability to mitigate necrotic enteritis caused by Clostridium perfringens. In the Landy medium, cellobiose was screened as the carbon source among glucose, mannose, arabinose, and maltodextrin, which significantly enhanced the anti-C. perfringens activity of the fermentation supernatant. When twelve amino acids, shikimic acid and two amino acid mixtures were added to cellobiose-supplemented Landy medium, the antibacterial activity was not enh

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12830197 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature