Synthetic zipper mediated pre-targeting system for near-infrared photoimmunotherapy.

ABSTRACT: Several strategies have gained attraction in cancer diagnosis and therapy, particularly to overcome the limitations associated with direct targeting approaches in radioimmunotherapy and photoimmunotherapy. Here, we have introduced a synthetic zipper-mediated pre-targeting system for near-infrared photoimmunotherapy (NIR-PIT) utilizing the IR700-conjugated Zip1-SNAP protein in combination with three scFv-ZIP2 fusion proteins targeting FOLR1, TROP2, and TF. In this two-step targeting approach, scFv-Zip2 proteins first bind to cancer-specific antigens, followed by hybridization with Zip1-SNAP-IR700 at cell surface via high-affinity zipper interaction. Upon NIR light irradiation, targeted cancer cells underwent selective phototoxicity in a concentration-dependent manner with IC₅₀ ra

SUBMITTER: Mohiuddin TM 

PROVIDER: S-EPMC12830222 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature