Imputation integrates single-cell and spatial gene expression data to resolve transcriptional networks in barley shoot meristem development.

ABSTRACT: Grass inflorescences are composite structures, featuring complex sets of meristems as stem cell niches that are initiated in a repetitive manner. Meristems differ in identity and longevity, generate branches or split to form flower meristems that finally produce seeds. Within meristems, distinct cell types are determined by positional information and the regional activity of gene regulatory networks. Understanding these local microenvironments requires precise spatio-temporal information on gene expression profiles, which current technology cannot achieve.Here we investigate transcriptional changes during barley development, from the specification of meristem and organ founder cells to the initiation of distinct floral organs, on the basis of an imputation approach integrating deep single-

SUBMITTER: Demesa-Arevalo E 

PROVIDER: S-EPMC12830361 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature