Ultra-sensitive nanostructured electrochemical immunosensor for selective monitoring of L-phenylalanine in phenylketonuria patients.

ABSTRACT: A low cost and rapid electrochemical immunosensor for direct screening of L-phenylalanine (L-Phe) using square wave voltammetry (SWV) is presented. Fern-like gold nanostructures were electro-deposited onto a screen-printed electrode (SPE) using chronoamperometry (CA), to enhance sensitivity and surface area. A novel light-driven surface functionalisation process was used to decorate the gold nanostructured SPE with target-specific antibody fragments and develop the L-Phe-specific immunosensor. The selectivity of the sensor was confirmed against tyrosine, tryptophan and D-phenylalanine, thus indicating the sensor's potential for use at points of care (POC) without interference from structurally related biomolecules. The sensor demonstrated high sensitivity across the full clinical range (12

SUBMITTER: Houston RL 

PROVIDER: S-EPMC12830426 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature