Effects of intravenous lipid emulsions on Jurkat cells assessed using label-free deformability cytometry.

ABSTRACT: Intravenous lipid emulsions (ILEs) are routinely used in clinical nutritional support and exert immunomodulatory effects, although they remain poorly understood. This is particularly relevant for cell-based immunotherapies, such as CAR-T therapy, where interactions between immune cells and circulating lipids may influence treatment efficacy. We investigated the effects of two commonly used ILEs (SMOFlipid and Omegaven) on Jurkat cells, using deformability cytometry (DC). Beyond assessing cell mechanics, we extended DC with two novel, label-free approaches: deep neural network-based image classification for cell state evaluation and morphological analysis for lipid droplet detection.Our results show that ILE composition strongly affects cytotoxicity. Omegaven, rich in omega-3 polyunsaturate

SUBMITTER: Fajdiga Lebar L 

PROVIDER: S-EPMC12830582 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature