Femtogram-level VEGF detection via PEG-directed gold nanostructured electrochemical immunosensor.

ABSTRACT: This study presents a novel electrochemical immunosensor designed for the highly sensitive detection of vascular endothelial growth factor (VEGF). The sensor utilizes gold nanostructures grown on fluorine-doped tin oxide (FTO) electrodes through a polyethylene glycol (PEG)-mediated electrochemical process. Fabrication involves a two-step immobilization strategy, combining physical vapor deposition (PVD) of a gold thin film with thermal annealing. The nanostructured electrode is subsequently modified electrochemically via PEG mediation, followed by antibody functionalization to enhance binding specificity. PEG-mediated control over nanostructure growth yields irregular short nanorods and leaf-like dendrites, significantly enhancing sensor performance. Extensive electrochemical analyses, inc

SUBMITTER: Mirmohamadi B 

PROVIDER: S-EPMC12830629 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature