BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress.

ABSTRACT: DNA G-quadruplexes (G4s) spontaneously arise in guanine-rich regions such as telomeres. Although G4s regulate essential biological process, their persistence can interfere with DNA metabolism and induce replication stress (RS). Helicases such as BLM and FANCJ play key roles in resolving G4 structures to maintain genome stability. Our investigation delves into how BLM and FANCJ respond to telomeric RS induced by the telomeric G4 ligand, RHPS4. To examine their individual and combined functions, we employed CRISPR/Cas9 to deplete BLM and siRNA to silence FANCJ in U251MG glioblastoma cells. Our results revealed a functional interplay between the two helicases: RHPS4 treatment led to increased FANCJ protein levels and telomeric recruitment in both wild-type and BLM^-/- cells. Notably

SUBMITTER: Bonanni L 

PROVIDER: S-EPMC12830668 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature