Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway.

ABSTRACT: Osteogenic differentiation is a critical biological process for bone formation and regeneration. Exosomes, as extracellular vesicles, play vital roles in intercellular communication by transferring bioactive molecules. This study aimed to investigate the role and mechanism of exosomal miRNAs derived from human bone mesenchymal stem cells (BMSCs) in regulating osteogenic differentiation of human osteoblast cells (hFOB1.19). BMSCs were characterized by assessing multipotential differentiation capacity. Exosomes from BMSCs were isolated and identified. miRNA qPCR array analysis was used to screen differentially expressed miRNAs in BMSCs-derived exosomes. Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining were performed to evaluate osteogenic differentiation. Reverse transcription qu

SUBMITTER: Liu L 

PROVIDER: S-EPMC12830772 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature