Disulfiram activates autophagy via proteasome inhibition and c-Fos/beclin-1 upregulation, synergizing with chloroquine.

ABSTRACT: Disulfiram (DSF), a clinically approved anti-alcoholism drug, exerts anti-tumor activity through its copper metabolite CuET by inhibiting the ubiquitin-proteasome system (UPS). However, its regulatory mechanisms on autophagy and potential for combination therapy remain to be clarified. Here, we revealed that DSF activates autophagy in colorectal cancer (CRC) cells via dual mechanisms: compensatory autophagy induction through proteasome inhibition by targeting the p97-NPL4 axis, and transcriptional upregulation of the autophagy-related gene BECN1 via FOS gene activation. Transcriptomic analysis identified that DSF enhances c-Fos expression, promoting c-Fos/AP-1 complex binding to the BECN1 promoter to drive beclin-1 expression. Furthermore, combining DSF with the autophagy inhibitor chloroq

SUBMITTER: Wang K 

PROVIDER: S-EPMC12830890 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature