A versatile platform for chemical engineering of exosomes empowered by ADP-ribosyl cyclases.

ABSTRACT: Cell-secreted exosomes have been emerging as an increasingly attractive form of nanomaterials for biomedical research. Various approaches have been established to genetically modify exosomes with proteins of interest for new and/or improved functions. However, equipping exosomes with diverse non-protein biomolecules remains largely dependent on random chemical conjugation or membrane insertion, hindering the application potential of exosomes. Herein, we develop a technology for site-specific functionalization of exosome with different synthetic groups by exploiting surface-expressed CD38, an ADP-ribosyl cyclase, and its covalent inhibitor derived from nicotinamide adenine dinucleotide (NAD⁺). The designed ADP-ribosyl cyclase-enabled exosomes (ARC Exos) carrying conjugated fluore

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12830964 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature