TTBK2-Driven Ciliogenesis Is Required for Intrinsic Neuronal Regeneration After Spinal Cord Injury.

ABSTRACT:

Background

The primary cilium (PC) is a pivotal organelle for neuronal signaling and development, while tau tubulin kinase 2 (TTBK2) is a key initiator of ciliogenesis. However, the role of TTBK2 in spinal neurons during spinal cord injury (SCI) and subsequent neural repair remains unclear.

Methods

We manipulated TTBK2 expression in spinal neurons using adenovirus-mediated overexpression and knockdown in vitro. Transcriptomic profiling (RNA-sequencing) and RT-qPCR were employed to explore the potential regulatory pathways at the molecular level. In vivo, Ttbk2^fl/fl-Rosa-CreERT2^+/- conditional knockout mice were subjected to a spinal cord hemisection model. Behavioral assays, immunofluorescence, and biotinylated dextran amine (BDA) tracing were conducted

SUBMITTER: Zhang R 

PROVIDER: S-EPMC12831131 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature