Combined Mouse Retinal Optoretinography/Electroretinography System to Study Light-Evoked Responses in Animal Models of Retinal Degeneration.

ABSTRACT:

Purpose

To study the correlation between optoretinogram (ORG) and the underlying phototransduction-initiated physiology, we irradiated mouse retinas with visible light centered at a 482-nm wavelength to induce bleach-initiated morphologic changes in the outer retinal layers, including photoreceptors and the retinal pigment epithelium (RPE).

Methods

A 482-nm light-emitting diode was used for short-pulse irradiation of the retina over a large field of view in the three groups of mice, including disease models, while acquiring optical coherence tomography (OCT) image sequences using a custom-built OCT system combined with a commercial electroretinogram (ERG) system. The visible light exposure was adjusted to vary the total light energy (number of photons) delivered to the retin

SUBMITTER: Jo HC 

PROVIDER: S-EPMC12831139 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature