Layer-Specific Proteomic Profiling of the Human Cornea Reveals Insights Into Structure and Biological Function.

ABSTRACT:

Purpose

Clinical proteomics enhances our understanding of biological functions and pathological processes. Since the localization of various protein clusters within the cornea is still unknown, a spatial model of the human corneal proteome was established.

Methods

In this study, successive layers of corneal tissue from five human corneas were ablated using a nanosecond mid-infrared laser, with ablation depth verified by optical coherence tomography. Each layer was analyzed by LC-MS/MS-based quantitative proteomics to generate a spatial map of the corneal proteome.

Results

A total of 4,454 proteins were identified. A clear distinction between proteome clusters reflecting the tissue layers within the cornea was observed. Various biological processes were localized in th

SUBMITTER: Schadwinkel HM 

PROVIDER: S-EPMC12831156 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature