Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering.

ABSTRACT: Novel cutting-edge technologies for plastid genetic engineering have a great potential in agriculture. Genetic engineering of the plastid genome (plastome) can be performed using both conventional homologous recombination vectors, and novel episomal platforms that rely on synthetic plastomes (minisynplastomes) to express transgenes from a nonintegrating plasmid. Evaluating the potential risk of horizontal gene transfer (HGT) is an important step for regulatory approval of environmental release of these novel genetic engineering tools. In particular, the endosymbiotic origin of plastids from a prokaryotic progenitor may increase the probability of HGT to the environmental microbial community. In this study, the naturally competent soil bacterium Acinetobacter baylyi has been used to test th

SUBMITTER: Vincent AG 

PROVIDER: S-EPMC12831460 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature