Virus-like particle (VLP)-based indirect ELISA (iELISA) for the detection of beak and feather disease virus (BFDV) antibodies.

ABSTRACT: Beak and feather disease virus (BFDV) poses a significant threat to avian biodiversity and global aviculture. Reliable serodiagnostic tools are critical for assessing host immune status and guiding disease management. The haemagglutination inhibition (HI) assay, although historically regarded as the gold standard, is limited by technical complexity and its reliance on seropositive Galah erythrocytes, restricting broader application. This study describes the development and validation of a recombinant BFDV capsid protein-based indirect enzyme-linked immunosorbent assay (iELISA) for detecting anti-BFDV antibodies using dried blood spots from multiple psittacine species. The assay demonstrated high sensitivity and strong analytical performance, employing virus-like particles (VLPs) recombinan

SUBMITTER: Dhar PK 

PROVIDER: S-EPMC12831674 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature