N-Glycosylation of Band 3 is essential for the binding of Ganoderma lucidum immunomodulatory protein rLZ-8.

ABSTRACT: Fungal immunomodulatory proteins (FIPs) are considered potential therapeutic candidates due to their immunomodulatory and anti-cancer activities. Meanwhile, they are capable of agglutinating mammalian red blood cells in a manner similar to lectins. Herein, GST-tagged rLZ-8, the first identified FIP in Ganoderma lucidum, was employed as bait to pull down its target proteins from mouse red blood cells. An important membrane protein, Band 3, was found to interact with rLZ-8. The result was confirmed by detecting the interactions of rLZ-8 with mBand 3 and hBand 3 heterologously expressed in HEK293 cells. Moreover, the interaction specifically required the sole N-glycosylation of Band 3 at its extracellular loop (between the seventh and eighth transmembrane helices), as evidenced by diminished

SUBMITTER: Diao W 

PROVIDER: S-EPMC12831678 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature