MTHFR 677C/T gene polymorphism and dietary habits: effects on trace element levels, amino acids, and biochemical parameters.

ABSTRACT:

Background

Previous research has identified that, in individuals with lower folate and/or vitamin B₁₂ levels, homocysteine is associated with specific elements, while amino acids are associated with folate, thereby influencing folate availability during supplementation. This study investigates the impact of the MTHFR 677C/T polymorphism on levels of these compounds in individuals with differing meat consumption preferences, non-vegetarians and vegetarians.

Methods

The study was conducted on 162 Croatian subjects. MTHFR gene polymorphism was determined by PCR-RFLP, elements by ICP-QQQ, amino acids by GC-MS, and biochemical parameters by chemiluminescent immunoassay and enzymatic methods. Differences between the groups were tested by ANOVA, while assoc

SUBMITTER: Orct T 

PROVIDER: S-EPMC12832494 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature