LncRNA PVT1 targets miR-143-3p to modulate endothelial cell function and thereby participate in deep vein thrombosis (DVT) of the lower limbs.

ABSTRACT: The aim of this study is to investigate the diagnostic value of long non-coding RNA PVT1 (LncRNA PVT1) in deep vein thrombosis (DVT) of the lower limbs, as well as its regulatory role in damaged endothelial cells. A total of 384 participants were enrolled, including 208 patients with DVT. 3mL venous blood were collected from each participant. Thrombosis location and severity were assessed via Doppler ultrasound or venography. An endothelial cell injury model was induced using 250 µM cobalt chloride (CoCl₂). Gene expression was evaluated using RT-qPCR, while cell function was assessed via CCK-8 assays and flow cytometry. Angiogenesis and inflammatory factor expression was measured by ELISA. Dual luciferase reporter assays validated gene-target relationships. LncRNA PVT1 was signi

SUBMITTER: Xiang J 

PROVIDER: S-EPMC12832578 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature