REST missense mutations reveal disrupted Re1 motif binding and co-repressor interactions in uterine fibroids.

ABSTRACT:

Introduction

The Re1-Silencing Transcription Factor (REST) is a master regulator of gene silencing, orchestrating transcriptional repression by tethering chromatin-modifying co-repressors to the Re1 motif of target genes. While REST is recognized as a sentinel of cellular identity, its role in uterine fibroids (UF) remains unclear. This study aims to investigate how structural perturbations in REST may compromise its regulatory function and contribute to altered transcriptional control in fibroid biology.

Methods

A deep structural interrogation of REST was performed through expansive in silico analysis of 938 missense SNPs. Evolutionary conservation was assessed across ten primate species to identify structurally disruptive variants. Structural modelling, protein-prot

SUBMITTER: Sriram S 

PROVIDER: S-EPMC12832642 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature