RNA transcripts in salivary extracellular vesicle cargo isolated from aged populations.

ABSTRACT:

Introduction

Human saliva contains numerous factors, including DNA, RNA, and protein, that may reflect the health status of the individual. Many of these factors are contained within extracellular vesicles (EVs). The contents of EVs are thought to mirror the cytoplasm of the cell of origin, providing insight into the health of the cell. We investigated the RNA content from EVs isolated from saliva (salEVs) to determine if we could detect transcripts associated with neurodegenerative conditions.

Methods

We characterized the RNA cargo of salEVs isolated from individuals over the age of 65 with normal cognition. The salEV RNA content was analyzed by RNA-seq and NanoString miRNA analysis.

Results

We found approximately 48.4% of the reads mapped to the human genome, with t

SUBMITTER: Wen S 

PROVIDER: S-EPMC12832735 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature