Factors that determine cell fate in mitotically arrested cancer cells.

ABSTRACT:

Introduction

Cancer cells display a high degree of heterogeneity in their responses to mitotic arrest, from apoptosis during mitosis to surviving mitotic failure and continuing to progress through the cell cycle. Thus, understanding the basis for this variation may prove valuable for developing more effective chemotherapeutic strategies.

Methods

A combination of biochemical and long-term live cell imaging approaches were applied to determine whether inhibition of Phosphoinositide 3-kinase (PI3K) signaling affected apoptosis in cancer cells arrested in prometaphase with a Kinesin Spindle Protein (KSP) inhibitor.

Results

Dual inhibition of KSP and PI3K signaling induced apoptosis more effectively than mitotic arrest or PI3K pathway inhibition alone. Live cell imaging wi

SUBMITTER: Ashraf N 

PROVIDER: S-EPMC12832742 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature