Life on the rocks: unexpected enzyme activity of the extremophilic black fungus Knufia chersonesos.

ABSTRACT: Knowledge about extremophile organisms and their survival strategies could be of great value for various industrial applications including biological plastics recycling. The black fungus Knufia chersonesos inhabits extreme environments such as rocks and therefore produces specific enzymes that function under harsh conditions. A cutinase (Kc_Cut) and a lipase (Kc_Lip) identified via proteomics-based screening in the secretome of K. chersonesos grown on poly (butylene-adipate-co-terephthalate) (PBAT) as carbon sources were recombinantly expressed in Komagataella phaffii and Trichoderma reesei, respectively, for further characterization. The purified enzymes showed a specific activity of 83 ± 1 and 0.23 ± 0.02 U mg^-1 on para-nitrophe

SUBMITTER: Mihalyi S 

PROVIDER: S-EPMC12832766 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature