Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cells.

ABSTRACT:

Background

The intestinal wall is the most commonly used substitution material in reconstructive urology. However, this strategy is associated with many complications. Tissue engineering techniques can replace the intestinal wall with an in vitro-created urinary tract substitute. Biomaterial seeding with urothelial cells (UCs) would increase the success of the regeneration process. The problem in many patients requiring urinary reconstruction is the lack of autologous UCs. This study aimed to compare the differentiation potential of porcine mesenchymal adipose tissue-derived stromal/stem cells (AD-MSCs) and progenitor buccal epithelial cells (pBECs) toward UCs. UCs, AD-MSCs, and BECs were isolated from tissues harvested from domestic pigs. The conditioned medium was collecte

SUBMITTER: Buhl M 

PROVIDER: S-EPMC12832818 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature