Single-cell transcriptomics reveals a novel mechanism of RDH16 regulating immune infiltration in hepatocellular carcinoma.

ABSTRACT:

Introduction

Hepatocellular carcinoma (HCC) exhibits pronounced intratumoral heterogeneity and a complex immune microenvironment, which together limit therapeutic efficacy. Identifying actionable biomarkers and mechanisms of immune modulation remains critical for improving patient outcomes.

Method

We integrated single-cell RNA sequencing data with bulk transcriptomic datasets to comprehensively characterize tumor cell heterogeneity and immune landscape features in HCC. Associations between RDH16 expression and clinicopathological characteristics were evaluated, and in vitro functional assays were conducted. Mendelian randomization analyses were performed to assess causal relationships.

Results

RDH16 expression was significantly reduced in HCC tissues compared with non

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12832884 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature