FACS-based genome-wide CRISPR screening platform identifies modulators of CD47.

ABSTRACT:

Background

CD47 is a key innate immune checkpoint that enables tumor cells to evade macrophage-mediated clearance.

Methods/results

To systematically identify genetic regulators of CD47 surface expression, we performed FACS-based genome-wide CRISPR screens in three murine cancer cell lines B16 (melanoma), MC38 (colon adenocarcinoma), and EMT6 (breast carcinoma).

Results

Comparative analysis of cells with high or low CD47 surface expression using DrugZ revealed CD47 itself as the top hit, validating the screens. Notably, DNAJC13 emerged as a consistent and robust regulator of CD47 expression across all three cell lines. Functional validation using DNAJC13-knockout cells confirmed a significant reduction in CD47 surface levels. Furthermore, in co-culture assays with macr

SUBMITTER: Yin L 

PROVIDER: S-EPMC12832931 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature